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Application of Nanotechnology in Cancer Therapy and Imaging

Dr. Wang is Research Associate, Department of Hematology and Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA.
Dr. Yang is Associate Professor, Department of Surgery, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA.
Dr. Chen is Associate Professor, Department of Hematology and Oncology, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA.
Dr. Shin is Professor, Department of Hematology and Oncology and Otolaryngology; Director, Clinical and Translational Cancer Prevention Program; Co-Director, Translational Lung Aerodigestive Tract Malignancies Program; and Associate Director of Academic Development, Winship Cancer Institute, Emory University School of Medicine, Atlanta, GA.

Published online through CA First Look at http://CAonline.AmCancerSoc.org.
Disclosures: Dr. Shin was supported by NCI P50 CA128613, R01 CA 112643, U01 CA 101244, and U54 CA 119338. Dr. Shin and Dr. Chen both received the Georgia Cancer Coalition Distinguished Scholar Award.

	ABSTRACT

TOP ABSTRACT INTRODUCTION NANOPARTICLES FOR TUMOR... MULTIFUNCTIONAL NANOPARTICLES... IMPLICATIONS AND FUTURE... ACKNOWLEDGEMENT REFERENCES

 
Recent developments in nanotechnology have provided researchers with new tools for cancer imaging and treatment. This technology has enabled the development of nanoscale devices that can be conjugated with several functional molecules simultaneously, including tumor-specific ligands, antibodies, anticancer drugs, and imaging probes. Since these nanodevices are 100 to 1,000-fold smaller than cancer cells, they can be easily transferred through leaky blood vessels and interact with targeted tumor-specific proteins both on the surface of and inside cancer cells. Therefore, their application as cancer cell-specific delivery vehicles will be a significant addition to the currently available armory for cancer therapeutics and imaging.

	INTRODUCTION

TOP ABSTRACT INTRODUCTION NANOPARTICLES FOR TUMOR... MULTIFUNCTIONAL NANOPARTICLES... IMPLICATIONS AND FUTURE... ACKNOWLEDGEMENT REFERENCES

 
Cancer is one of the major causes of mortality in the United States, and the worldwide incidence of cancer continues to increase. The most common cancer treatments are limited to chemotherapy, radiation, and surgery. Frequent challenges encountered by current cancer therapies include nonspecific systemic distribution of antitumor agents, inadequate drug concentrations reaching the tumor, and the limited ability to monitor therapeutic responses. Poor drug delivery to the target site leads to significant complications, such as multidrug resistance.

Greater targeting selectivity and better delivery efficiency are the 2 major goals in the development of therapeutic agents or imaging contrast formulations. Ideally, a therapeutic drug would be selectively enriched in the tumor lesions with minimal damage to normal tissues. A rational approach to achieve these goals is to conjugate therapeutic drugs with monoclonal antibodies (mAbs) or other ligands that selectively bind to antigens or receptors that are usually abundantly or uniquely expressed on the tumor cell surface. Several ligand-targeted therapeutic strategies, including immunotoxins, radioimmunotherapeutics, and drug immunoconjugates, are being developed. Although these conjugated agents have demonstrated promising efficacy compared with conventional chemotherapy drugs in preclinical and clinical trials,¹ limitations in their delivery efficiency and specificity remain. For example, in vivo studies have shown that only 1 to 10 parts per 100,000 of intravenously administered mAbs, therapeutic, or imaging agents can reach their parenchymal targets.^2,3

At present, noninvasive imaging approaches, including x-ray–based computer-assisted tomography (CT), positron emission tomography (PET), single-photon emission tomography, and magnetic resonance imaging (MRI), are used as important tools for detection of human cancer.^4–9 The development of tumor-targeted contrast agents based on a nanoparticle formulation may offer enhanced sensitivity and specificity for in vivo tumor imaging using currently available clinical imaging modalities.

By applying a vast and diverse array of nanoparticles, whose design derives from the engineering, chemistry, and medicine fields, to molecular imaging and targeted therapy, cancer nanotechnology promises solutions to several of the current obstacles facing cancer therapies. Nanoparticles have a mesoscopic size range of 5 to 200 nm, allowing their unique interaction with biological systems at the molecular level. As a result of their material composition, nanoparticles are capable of self-assembly and maintaining stability and specificity, which are crucial to drug encapsulation and biocompatibility. Recent progress in cancer nanotechnology raises exciting opportunities for personalized oncology in which diagnosis and treatment are based on the molecular profiles of individual patients.

In this review, we will address first the types and characteristics of nanoparticles; second, how nanoparticles can be used as drug delivery systems and imaging devices to increase the efficacy per dose of therapeutic or imaging contrast agents; and last, how nanoparticles will be further developed to improve their functionality in cancer treatment and imaging.

	NANOPARTICLES FOR TUMOR TARGETING AND DELIVERY

TOP ABSTRACT INTRODUCTION NANOPARTICLES FOR TUMOR... MULTIFUNCTIONAL NANOPARTICLES... IMPLICATIONS AND FUTURE... ACKNOWLEDGEMENT REFERENCES

 
    Types of Nanoparticles as Drug Delivery Systems
Nanoparticles can consist of a number of materials, including polymers, metals, and ceramics. Based on their manufacturing methods and materials used, these particles can adopt diverse shapes and sizes with distinct properties. Many types of nanoparticles are under various stages of development as drug delivery systems, including liposomes and other lipid-based carriers (such as lipid emulsions and lipid-drug complexes), polymer-drug conjugates, polymer microspheres, micelles, and various ligand-targeted products (such as immunoconjugates).^{10,11,12–13}

    Liposomes and Other Lipid-based Nanoparticles
Liposomes are self-assembling, spherical, closed colloidal structures composed of lipid bilayers that surround a central aqueous space. Liposomes are the most studied formulation of nanoparticle for drug delivery (Table 1). Several types of anticancer drugs have been developed as lipid-based systems by using a variety of preparation methods. Liposomal formulations have shown an ability to improve the pharmacokinetics and pharmacodynamics of associated drugs.¹ To date, liposome-based formulations of several anticancer agents (Stealth liposomal doxorubicin [Doxil], liposomal doxorubicin [Myocet], and liposomal daunorubicin [DaunoXome]) have been approved for the treatment of metastatic breast cancer and Kaposi's sarcoma.^{2,14,15,17,30,31–32}

    Polymeric Nanoparticles
To reach the targeted tumor tissue, nanoparticles must be able to stay in the bloodstream for considerable lengths of time without being eliminated. Nanoparticles with no surface modification are usually caught by the MPS, primarily the liver and spleen, during circulation, depending on their size and surface characteristics.¹¹ To overcome this problem, nanoparticles can be coated with hydrophilic polymers. Coating can efficiently protect nanoparticles from capture by macrophages.^48,49–50 The increased hydration also helps nanoparticles to be more water soluble and less sensitive to enzymatic degradation, therefore enhancing biocompatibility.^50,51

During the past decade, the application of polymer-based drug delivery systems in oncology has grown exponentially with the advent of biodegradable polymers. In these polymers, drugs are either physically dissolved, entrapped, encapsulated, or covalently attached to the polymer matrix.⁵² The resulting compounds may have different structures, including micelles and dendrimers. Both natural (albumin, chitosan, heparin, etc.) and synthetic (poly-L-lactide, poly-[L-glutamate], poly-[D,L-lactide-co-glycolide], PEG, etc.) biodegradable polymers are being exploited as drug delivery systems.

Recently, a nanoparticle formulation of paclitaxel bound to albumin (Abraxane or ABI-007) was approved for the treatment of metastatic breast cancer.^53,54–55 In a Phase III clinical trial, ABI-007 showed greater therapeutic efficacy and increased response compared with free paclitaxel.^53,55 Currently, more than 10 formulations of anticancer polymeric nanoparticles have entered clinical development, including paclitaxel poliglumex (Xyotax),^56,57 N-(2-hydroxypropyl) methacrylamide (HPMA) copolymer-camptothecin (MAG-CPT),^58,59 and HPMA-DOX (PK1)⁶⁰ (Table 2). In Phase I/II clinical trials, HPMA-DOX showed a 4 to 5-fold reduction in anthracycline-related toxicity.^63,64,76 At DOX-equivalent doses of 80 to 320 mg/m², the drug still displayed significant antitumor activity in chemotherapy-refractory patients (including those with breast cancer).⁷⁶ A recent Phase III trial showed that paclitaxel poliglumex (Xyotax) was less toxic than free paclitaxel and could prolong the survival of non-small-cell lung cancer patients with poor performance status.^61,77 Also, paclitaxel poliglumex can be used as a novel radiation sensitizer.⁷⁸

    Passive Targeting
Passive targeting takes advantage of the inherent size of nanoparticles and the unique properties of tumor vasculature, such as the enhanced permeability and retention (EPR) effect and the tumor microenvironment.^{79,80,81–82} This approach can effectively enhance drug bioavailability and efficacy.

EPR Effect. Angiogenesis is crucial to tumor progression. Angiogenic blood vessels in tumor tissues, unlike those in normal tissues, have gaps as large as 600 to 800 nm between adjacent endothelial cells.^18,83 This defective vascular architecture coupled with poor lymphatic drainage induces the EPR effect,^{83,84,85–86} which allows nanoparticles to extravasate through these gaps into extravascular spaces and accumulate inside tumor tissues⁸⁷ (Figure 1). Dramatic increases in tumor drug accumulation, usually of 10-fold or greater, can be achieved when a drug is delivered by a nanoparticle rather than as a free drug.⁸⁸ However, the localization of nanoparticles within the tumor is not homogeneous. The factors that result in high concentrations of nanoparticles in one part of the tumor tissue but not in other parts are not well understood yet.⁸⁹ In general, the accumulation of nanoparticles in tumors depends on factors including the size, surface characteristics, and circulation half-life of the nanoparticle and the degree of angiogenesis of the tumor. Usually, less nanoparticle accumulation is seen in preangiogenic or necrotic tumors.¹⁸

View larger version (47K): [in this window] [in a new window] [PowerPoint slide for Educational Purposes]   FIGURE 1 Passive Tumor Targeting with Nanoparticle Drugs. Long-circulating therapeutic nanoparticles accumulate passively in solid tumor tissue by the enhanced permeability and retention effect. The hyperpermeable angiogenic tumor vasculature allows preferential extravasation of circulating nanoparticles.

    Active Targeting
The polymeric nanoparticles that have been tested clinically so far have mostly lacked a targeting moiety and instead rely mainly on the EPR effect of tumors, the tumor microenvironment, and tumor angiogenesis to promote some tumor-selective delivery of nanoparticles to tumor tissues. However, these drug delivery systems using a binary structure conjugate inevitably have intrinsic limitations to the degree of targeting specificity they can achieve. In the case of the EPR effect, while poor lymphatic drainage on the one hand helps the extravasated drugs to be enriched in the tumor interstitium, on the other hand, it induces drug outflow from the cells as a result of higher osmotic pressure in the interstitium, which eventually leads to drug redistribution in some portions of the cancer tissue.⁹³

An alternative strategy to overcome these limitations is to conjugate a targeting ligand or an antibody to nanoparticles. By incorporating a targeting molecule that specifically binds an antigen or receptor that is either uniquely expressed or overexpressed on the tumor cell surface, the ligand-targeted approach is expected to selectively deliver drugs to tumor tissues with greater efficiency (Figure 2). Such targeted nanoparticles may constitute the next generation of polymeric nanoparticle drug delivery systems. Indeed, several targeted polymeric nanoparticles are currently undergoing preclinical studies.^{65,77,94,95–96} One of these, HPMA copolymer-DOX-galactosamine (PK2, FCE28069), has progressed to a clinical trial. In this nanoparticle, galactosamine moieties bind to the asialoglycoprotein receptor on hepatocytes.^65,76 In a Phase I/II study, this targeted nanoparticle showed 12- to 50-fold greater accumulation than the free DOX in hepatocellular carcinoma tissue. Antitumor activity was observed in patients with primary hepatocellular carcinoma in this study.^65,76 These promising early clinical results suggest the potential of targeted polymeric nanoparticles as anticancer drug delivery systems. Lessons have also been learned from many of the early clinical studies. For example, the failure of HPMA conjugates of paclitaxel and camptothecin in Phase I clinical trials was reported. Such negative outcomes underline the importance of polymer-drug design.^66,97

View larger version (22K): [in this window] [in a new window] [PowerPoint slide for Educational Purposes]   FIGURE 2 Internalization of Nanoparticles via Receptor-mediated Endocytosis. Nanoparticle-conjugated tumor-specific ligands/antibodies bind to surface receptors, triggering nanoparticle internalization through an endosome-dependent mechanism. As the interior of the endosome becomes more acidic, drugs are released from the nanoparticle into the cytoplasm.

Choice of Targeting Ligand. One of the greatest challenges to the design of nanoparticles that can selectively and successfully transport drug to cancerous tissues is the choice of targeting agent(s). This strategy also relies on the ability of the targeting agent or ligand to bind the tumor cell surface in an appropriate manner to trigger receptor-mediated endocytosis. The therapeutic agent will thereby be delivered to the interior of the cancer cell.⁸⁵ A variety of tumor-targeting ligands, such as antibodies, growth factors, or cytokines, have been used to facilitate the uptake of carriers into target cells.^{90,92,99,100,101,102,103,104,105,106–107}

Ligands targeting cell-surface receptors can be natural materials like folate and growth factors, which have the advantages of lower molecular weight and lower immunogenicity than antibodies. However, some ligands, such as folate that is supplied by food, show naturally high concentrations in the human body and may compete with the nanoparticle-conjugated ligand for binding to the receptor, effectively reducing the intracellular concentration of delivered drug. Recent advances in molecular biology and genetic engineering allow modified antibodies to be used as targeting moieties in an active-targeting approach. MAbs or antibody fragments (such as antigen-binding fragments or single-chain variable fragments) are the most frequently used ligands for targeted therapies. Whole mAbs have 2 binding domains showing high binding avidity. The Fc domain of the mAb can induce complement-mediated cytotoxicity and antibody-dependent, cell-mediated cytotoxicity, leading to additional cell-killing effect. On the other hand, the Fc domain also initiates an immune response and can be rapidly eliminated in the circulation, resulting in decreased accumulation of targeted nanoparticles into cancer cells.¹³ Compared with whole mAbs, the use of antibody fragments as a targeting moiety can reduce immunogenicity and improve the pharmacokinetic profiles of nanoparticles.¹ For example, liposomes coupled with mAb fragments instead of whole antibodies showed decreased clearance rates and increased circulation half-lives, allowing the liposomes sufficient time to be distributed and bind to the targeted cells.^1,39 This strategy improved the therapeutic efficacy of immunoliposomal DOX targeted against CD19 on human B lymphoma cells in animal models.^1,39

    Reduction or Reversion of Multidrug Resistance
Drug resistance is one of the major obstacles limiting the therapeutic efficacy of chemotherapeutic or biologic agents. In the clinic, chemoresistance is defined as either a lack of tumor-size reduction or the occurrence of clinical relapse after an initial positive response to antitumor treatment. Drug resistance can be caused by (1) physiological barriers (noncellular-based mechanisms) or (2) alterations in the biochemistry of cancer cells (cellular mechanisms). First, noncellular drug-resistance mechanisms can be due to physiological barriers, which protect cancerous cells from drug-induced cytotoxicity. One of the most effective barriers in the body is the blood-brain barrier (BBB) that restricts the entry of anticancer agents to the brain from the periphery.¹⁰⁸ The low permeability of the BBB is mainly attributed to microvessel endothelial cells in the brain. These cells contain an extremely active efflux pump system, similar to that identified in tumor cells, which removes a large volume of agents from the brain to the blood. A recent study showed that ulex europeus agglutinin I-conjugated nanoparticles can effectively bypass the BBB.¹⁰⁹ The acidic environment in tumors can also result in resistance to basic drugs through neutralization. Further, high interstitial pressure and low microvascular pressure may also impede extravasation of drug molecules. Secondly, resistance of tumors to therapeutic intervention may be due to cellular mechanisms, such as altered activity of specific enzyme systems (for example, topoisomerase activity), altered apoptosis regulation, or increased drug efflux in malignant cells. Among these mechanisms, changes in the drug efflux pump are the best known and most extensively investigated. P-glycoprotein (p-gp), a product of the MDR1 gene, is a 170-kD transmembrane glycoprotein that functions as an efflux pump to remove drug out of cells, thus reducing the intracellular concentration of the drug. The p-gp pump usually recognizes substrate drugs and pumps them out of the cell as they pass through the plasma membrane. To date, several p-gp inhibitors have been investigated as potential anticancer agents. In preclinical studies, some of these p-gp inhibitors have shown the restoration of cancer-cell sensitivity to anticancer drugs. Unfortunately, when coadministered with anticancer agents, these inhibitors have generated considerable toxicity.^110,111

Given the enormous capacity of cancer cells to deploy various mechanisms to ensure their survival in the face of treatment with anticancer drugs, it is not surprising that promising strategies to inhibit drug resistance have proven difficult to translate into clinical success. Strategies for overcoming drug resistance should be based on new drug delivery systems, which will allow selective drug accumulation in tumor tissues, tumor cells, or even compartments of tumor cells. Nanoparticles are exemplars of such delivery systems, which aim to overcome both noncellular- and cellular-based drug resistance and to increase selectivity of drugs toward cancer cells while reducing their toxicity toward normal tissues.

By choosing an appropriate nanoparticle polymer carrier, it is possible to protect an antitumor drug from the acidic microenvironment it encounters before penetration into tumor cells. It is also expected that nanoparticles can bypass the p-gp efflux pump, leading to greater intracellular accumulation. For example, DOX-loaded poly (alkyl cyanoacrylate) nanoparticles were able to penetrate cells without being recognized by p-gp through forming an ion pair between degradation products and the drug.¹¹² A clinical study from Northfelt's group has shown that liposomal DOX is able to overcome drug resistance in AIDS-related Kaposi's sarcoma.^113,114 Another way to bypass the p-gp efflux pump is to deplete adenosine triphosphate (ATP), which is necessary for the ATP-dependent transporter to function properly. An ATPase inhibitor pluronic block copolymer (P85) has been shown to enhance the permeability of drugs through the BBB by inhibiting the p-gp drug efflux system.^115,116 This effect was attributed to the combination of ATP depletion and ATPase inhibition. A micellar formulation of DOX using P85 has shown more effective apoptosis in drug-resistant breast cancer cells.^115,116 Ligand-targeted strategies, especially those using receptor-targeting ligands, have also been applied to overcome drug resistance since these ligands are internalized via receptor-mediated endocytosis, bypassing the plasma membrane where p-gp primarily acts. As an example, folate receptor-targeted, pH-sensitive polymeric micelles containing DOX¹¹⁷ and transferrin-conjugated paclitaxel nanoparticles exhibited greater cytotoxicity than the respective free drugs in a drug-resistant model.¹¹⁸

	MULTIFUNCTIONAL NANOPARTICLES FOR TUMOR IMAGING

TOP ABSTRACT INTRODUCTION NANOPARTICLES FOR TUMOR... MULTIFUNCTIONAL NANOPARTICLES... IMPLICATIONS AND FUTURE... ACKNOWLEDGEMENT REFERENCES

 
Tumor imaging plays a key role in clinical oncology, with radiological examinations able to detect solid tumors, determine recurrence, and monitor therapeutic responses. Conventional tumor imaging approaches such as CT and MRI focus mainly on delineating morphological features of the tumor, tissue, and organs, such as the anatomic location, extent, and size of the tumor, at various levels of spatial resolution and contrast. Despite continuous improvements in spatial resolution with advanced imaging equipment, imaging modalities using nontargeted contrast agents such as CT and MRI have limited sensitivity and ability to provide specific and functional information on the disease, which is increasingly recognized to be an obstacle to earlier diagnosis and the monitoring of treatment responses.

Recent advances have stimulated the emergence of the new field of "molecular imaging," which focuses on visualizing or imaging biological events and processes in living systems, including patients.^9,119 Current molecular imaging approaches, including PET, single-photon emission tomography, and optical imaging including fluorescence-mediated tomography and near-infrared fluorescence reflectance (NIRF) imaging, have shown a high sensitivity in noninvasive tumor imaging.^4,5,7 A commonly used PET imaging probe, ¹⁸F-labeled fluorodeoxyglucose (FDG), can only localize tumors by identifying cells in the body that have increased glucose uptake and metabolism, allowing for the detection of those tumors. However, it is not suitable for tumor types with a low glucose uptake. It is well recognized that the development of novel approaches for early cancer detection and effective therapy will significantly contribute to the improvement of patient survival. The development of nanoparticles as imaging contrast agents also makes it possible for the production of multifunctional nanoparticles with the capacity of targeted tumor imaging and delivery of therapeutic agents. In comparison with radioactive probes (ie, ¹⁸F-labeled FDG) used for PET imaging, nanoparticles have both greater surface areas and more functional groups that can be linked with multiple diagnostic and therapeutic agents.

One molecular imaging strategy to improve the specificity of cancer detection is target-specific imaging of biomarker molecules specifically produced by cancer cells, coupled with imaging probes guided by ligands that can recognize and interact with target molecules. Recently, tumor-targeted optical, radioactive, or magnetic probes have been generated and their feasibility examined in animal tumor models and in very limited clinical studies.^{120,121,122,123–124} However, to develop this promising tumor imaging approach and eventually translate it to clinical applications, several important issues have to be addressed, including (1) identification of a target molecule that is highly expressed in most tumor cells, but is found at a low or undetectable level in normal cells; (2) production of stable and high-affinity targeting molecules in large enough quantities for potential in vivo imaging in animal models and eventual clinical use; (3) development of imaging probes emitting a strong enough signal to improve the sensitivity of cancer detection, but with a low toxicity to normal organs and tissues; and (4) increase in retention time of the targeted imaging probes in blood circulation, allowing for their accumulation to sufficient levels in the tumor mass.

Advances in nanotechnology have shown the promise of nanoparticles for tumor-targeted drug delivery and noninvasive tumor imaging.^{79,121,125,126–127} With unique pharmacokinetics, nanoparticles with sizes between 10 to 100 nm have a prolonged circulation time since they are usually not taken up by the MPS within the liver or excreted by the kidney, common limitations to the delivery of small molecular imaging agents or drugs.¹²⁸ Such nanoparticles can navigate the vasculature and cross barriers through small capillaries into tumor cells. Extensive research has shown that nanoparticles in the above size range accumulate preferentially in tumor sites through the EPR effect associated with tumor growth.^79,128 Moreover, the optical and electronic properties and biodistribution of many nanoparticles are often dependent on size. Nanoparticles of specific sizes can be synthesized under controlled conditions to obtain the desired optical and magnetic properties and levels of therapeutic agents attached to the particles.^79,121 These properties offer the opportunity to design "smart" nanoparticles, including target-specific contrast agents, multimodality imaging probes, or even multifunctional reagents for simultaneous imaging and treatment.

    Quantum Dot Nanoparticles
Semiconductor quantum dots (QDs) are nanometer-scale, light-emitting particles with unique optical and electronic properties such as size-tunable light emission, improved signal brightness, enhanced stability of the fluorescent signal, and the ability to simultaneously excite multiple fluorescent colors.¹²⁹ These properties are most promising for improving the sensitivity of molecular imaging and quantitative cellular analysis by 1 to 2 orders of magnitude. Nie et al first reported that it is feasible to simultaneously target and image prostate tumors in living animal models using bioconjugated, prostate membrane antigen-targeted QDs.⁷⁹ This new class of QD conjugate contains an amphiphilic triblock copolymer layer for in vivo protection and multiple PEG molecules for improved biocompatibility and circulation, making it highly stable and able to produce bright signals. Another advantage is that QD probes emitting at different wavelengths can be used together for imaging and tracking multiple tumor markers simultaneously, potentially increasing the specificity and sensitivity of cancer detection.

Recently, QDs producing NIRF signals have been developed.^130,131 NIRF light penetrates much more deeply into tissues compared with visible fluorescence and allows for the detection of signals inside animals, as compared with visible fluorescent signals, which can only pass through several millimeters in the tissues (Figure 3). A major advantage of NIRF QDs is that their emission is well beyond the spectral range of the fluorescence signal produced by blood and tissues (autofluorescence), resulting in imaging with a high signal-to-background ratio.¹¹⁹ Detection of QD NIRF signals in sentinel lymph nodes within large animals in real time has been demonstrated.¹³⁰ Therefore, QDs are excellent optical imaging nanoprobes for evaluating the specificity of tumor-targeting ligands in vitro in tumor cells and in vivo in animal tumor models. Sensitive real-time detection of tissue distribution of targeted QDs is also possible using the NIRF optical imaging system after systemic delivery. However, since cadmium is the main component of most QDs, there is some concern over their potential toxicity, making the feasibility of using these QDs for future clinical application still undetermined.

View larger version (112K): [in this window] [in a new window] [PowerPoint slide for Educational Purposes]   FIGURE 3 Optical Imaging of Peritoneal Tumor Metastases Using Quantum Dots. Mouse mammary tumor 4T1 cells stably transfected with a firefly luciferase gene were incubated with Tat peptide-labeled quantum dots (emission 650 nm) for 2 hours before injection into mouse peritoneal cavity. Eight days later, bioluminescence imaging was performed on the mouse with a Kodak in vivo FX imaging system, and tumor locations (arrows) were determined. Optical imaging of peritoneal cavity confirmed the tumor lesions (arrows).

In recent years, significant efforts have been made to develop target-specific MRI contrast agents based on the formulation of IO nanoparticles. IO nanoparticles conjugated with ligands targeting cell surface markers such as MUC1, vβ3 integrins, Her-2/Neu, or folate receptor^{121,131,141,142,143–144} have been reported. However, several obstacles remain to be overcome. One of the major challenges is to develop a surface coating material that can not only stabilize the nanoparticle, but also provide active functional groups for controllable bioconjugation of "probe" ligands. For the specific needs of imaging applications in vivo, IO nanoparticles with a small size but high mass-magnetization value are desirable. For the specific purpose of cell targeting, activation of the particle surface for easy conjugation with biomolecules is essential. Because IO nanoparticles have low toxicity and a large surface area for carrying drugs, several studies have explored the feasibility of their use for the delivery of anticancer drugs. Indeed, the feasibility of simultaneous tumor MRI and drug delivery using vβ3 integrin-targeted multifunctional polymeric micelles containing DOX and a cluster of SPIO nanoparticles has been demonstrated.¹⁴⁵ In addition to chemotherapy drugs, IO nanoparticles for in vivo delivery of small interfering ribonucleic acids (siRNAs) have been developed in animal tumor models. SiRNAs can inhibit the expression of genes that are important for resistance to drug treatment by specifically binding to the target message RNAs, leading to their degradation. Such dual-purpose probes are capable of delivery of survivin siRNA, which targets an antiapoptotic protein that is upregulated in cancer cells, for cancer therapy as well as for simultaneous tumor imaging using MRI and NIRF imaging.¹²⁶

Recent efforts also focus on the development of ultrasensitive magnetic nanoprobes for tumor imaging. Using magnetism-engineered iron oxide nanoprobes that are conjugated with HER-2 antibodies, Lee et al showed an enhanced sensitivity of MRI for the detection of HER-2 expressing cancer in an animal model compared with that of commonly used SPIO probes.¹²¹ This new generation of magnetic nanoparticles should provide us with a powerful contrast agent for cancer detection.

	IMPLICATIONS AND FUTURE DIRECTIONS

TOP ABSTRACT INTRODUCTION NANOPARTICLES FOR TUMOR... MULTIFUNCTIONAL NANOPARTICLES... IMPLICATIONS AND FUTURE... ACKNOWLEDGEMENT REFERENCES

 
Cancer is known to develop via a multistep carcinogenesis process and to progress using several complex survival mechanisms, such as self-sufficiency in growth signaling, insensitivity to growth inhibitory signals, evasion of apoptosis, limitless replicative potential, sustained angiogenesis, and tumor invasion and metastasis.¹⁴⁶ To date, cancer treatments are performed on the basis of clinical and pathologic staging that is determined using morphologic diagnostic tools, such as conventional radiological and histopathological examinations. However, even patients suffering from cancers of the same cellular type and clinical stage respond to the same conventional treatment modalities differently and, ultimately, with variations in survival rate. This implies that cancer-associated events are unique in each patient.

Recent advances in molecular, biological, and genetic diagnostic techniques have begun to explore cancer-associated biomarkers and their implication for the development and progression of cancer and to reveal that cancer is controlled by complex multifactorial mechanisms rather than a single factor.¹⁴⁷ Molecularly targeted therapy is a recent introduction acknowledging our increased understanding of these cancer behaviors at the molecular level. Success of targeted therapies depends on expression of the targeted molecules, which can also serve as cancer-specific biomarkers.¹⁴⁸ Assays to accurately and quickly quantify several cancer-related biomarkers simultaneously on single tumor sections or small tumor specimens will be enabled by virtue of advances in nanotechnology.^149,150 For example, the use of conjugated QDs potentially allows 5 cancer-related proteins to be detected on the same tissue section.¹⁵¹

In addition to ex vivo analysis for the detection of early cancer and profiling of molecular biomarkers, in vivo imaging of cancer using several types of nanoparticles has also been investigated together with the progression of nanoscale drug delivery systems.⁷⁹ The development of multifunctional nanoparticles may contribute significantly to the realization of individualized therapy for cancer. Ideally, for constructing multifunctional nanoparticles, an appropriate combination of agents (therapeutic agent and targeting moiety) will be chosen based on accurate biological information within the tumor (molecular biomarker profiling of the patient) with imaging material attached on the nanoparticle surface (Figure 4). Nanoparticles may eventually be capable of detecting malignant cells (active-targeting moiety), pinpointing and visualizing their location in the body (real-time in vivo imaging), killing the cancer cells with minimal side effects by sparing normal cells (active targeting and controlled drug-releasing system), and reporting back that their payload has accomplished its mission (monitoring treatment effects in real time).

View larger version (48K): [in this window] [in a new window] [PowerPoint slide for Educational Purposes]   FIGURE 4 Multifunctional Nanoparticle. The multifunctional nanoparticle has the capability to simultaneously carry therapeutic agents, targeting molecules such as conjugated antibodies or other recognition agents, and imaging signal contrast agents. The nanoparticle can be used for specific delivery of anticancer agents, detection of circulating cancer cells, and monitoring of treatment effects in real time.

With this promising progress in the development of nanotherapeutic and imaging approaches to cancer detection and treatment, it is imperative to have a better understanding of the basic principles involved in designing and applying nanoparticles for diagnosis, treatment, or the combination of imaging and therapeutics in different clinical situations. There are certain critical questions that need to be addressed in the rational design and application of nanoparticles before further clinical development, such as how the association or conjugation of a therapeutic agent to ligand or carrier changes the pharmakinetics, biodistribution, and side effects of the nanotherapeutic drugs; how the safety profile of nanoparticles changed after conjugation, such as coating with QDs; how we can minimize the potential toxicity of polymeric nanoparticles that is inherent from the accumulation of a nonbiodegradable polymer with a size over the renal threshold³¹; and how side effects resulting from the ability of nanoparticles to cross the BBB can be prevented or diminished. These questions are critically important and hitherto understudied. The answers will certainly lead to more rational design of optimized nanoparticles with improved selectivity, efficacy, and safety. Attracted by the rapid and promising progress in nanotechnology, physicists, chemists, engineers, biologists, and clinicians will continue to challenge themselves to develop novel and efficacious nanosystems for the diagnosis and treatment of cancer.

	ACKNOWLEDGEMENT

TOP ABSTRACT INTRODUCTION NANOPARTICLES FOR TUMOR... MULTIFUNCTIONAL NANOPARTICLES... IMPLICATIONS AND FUTURE... ACKNOWLEDGEMENT REFERENCES

 
We thank Dr. Anthea Hammond for her assistance in critical reading and editing of the manuscript.

	REFERENCES

TOP ABSTRACT INTRODUCTION NANOPARTICLES FOR TUMOR... MULTIFUNCTIONAL NANOPARTICLES... IMPLICATIONS AND FUTURE... ACKNOWLEDGEMENT REFERENCES

 

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